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Ds virulent forms and can be local reactions at the usedDs virulent forms and can be local reactions at the used in immunodeficient hosts; not vaccine site) are required heat-labile to optimally trigger the adaptive immune system and generate long-term immunity; do not give rise to cytotoxic T cells; poor induction of mucosal immunity; difficult to produce in a scalable setting; quality and
[21] has not been so encouraging . the antigenic/therapeutic protein in vivo[21] has not been so encouraging . the antigenic/therapeutic protein in vivo and to stimulate [25] potent specific humoral and cellular immune responses . RNA viral vectors, such as retrovirus and lentivirus, allow long-term expression of the transgene, while DNA viral vectors allow expression in episomal form. Viral
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